Reporter

Part:BBa_K3170999

Designed by: Haodong Yan   Group: iGEM19_LZU-CHINA   (2019-10-16)
Revision as of 15:01, 21 October 2019 by Studentwang (Talk | contribs)

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FRET system for detecting TEVp activity

We constructed a FRET system for the detection of TEVp by the CFP-target sequence-YFP. When TEVp was expressed, the FRET system showed fluorescence transfer; when TEVp was not expressed, there was no fluorescence transfer. Finally, the fluorescent color expressed by the FRET system can judge the activity of TEVp.

Experimental Results

T--LZU-CHINA--Ras.png
T--LZU-CHINA--Flu.png
  • Imaging begins approximately 2 hours after the media is replaced with a fluorescent imaging medium. Get images every 1 hour. SW1990 cells were observed using 440 nm excitation light, and the cells turned from cyan to yellow.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None