Part:BBa_K3170602

OR Boolean logic gate

Compotent of the protease-protease regulation system

One of the logic gates of the core protein pathway designed using Boolean logic. We removed the target sequence of tobacco etch virus protease (TEVp), the target sequence of tobacco vein vein virus protease (TVMVp) and YFP on the right side of the CFP with the terminator removed, and combined them into OR Boolean logic gates.
In the OR logic gate, when both TEVp and TVMVp proteases are absent, FRET occurs, and the fluorescence emitted by the CFP absorption excitation light is absorbed by YFP, and the YFP emits yellow fluorescence. When any one of the proteases or both proteases is present, the target sequence of one of the proteases or the two proteases is cleaved, and the CFP is separated from the YFP. The two are far apart, failing to perform FRET, and YFP does not emit light. The CFP absorbs the excitation light and emits cyan fluorescence.
For OR logic gates, there are two inputs: the presence or absence of two proteases, TEVp and TVMVp, when the protease is present, ie the input value is 1, otherwise 0; there is an output: CFP luminescence or not, when CFP illuminates, The output is 1, otherwise it is 0.

Experimental Results

OR logic gate. The reporter was used at a concentration of 150 ng, the fluorescence value was measured at 515 nm, and the ordinate was the normalized fluorescence intensity value. The or logic gate pathway shows that when TEVP and TVMVP are present alone, the fluorescence value at 515 nm is high, and when both are present, the fluorescence value is very low, indicating that two proteins are required to function in the pathway alone.

Sequence and Features

Assembly Compatibility: