Coding

Part:BBa_K3286106

Designed by: Robert Hooftman   Group: iGEM19_Wageningen_UR   (2019-10-15)
Revision as of 14:32, 21 October 2019 by Roberthooftman (Talk | contribs)


Shorter version of PD1764

This protein is a shorter (sh) version of BBa_K3286105. Due to isolation troubles, the first 20 amino acids have been removed from this protein. The part contains a chitin-binding domain that is essential for chitin binding capacities [1].

[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016.


Functional Characterization

Figure 1: Protein concentration in supernatant in ug/ml. 55% of PD1764sh has bound to chitin in this assay. The influence of chitin on protein concentration in general is negligible, as the BSA sample indicates

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