Part:BBa_J10050:Experience

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UNIQc53a6d34c743b9c1-partinfo-00000000-QINU

•••• ETH Zurich 2014

Characterization of weak promoter (BBa_J23113) and weak RBS (BBa_B0031) Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10050 would result in the least amount of expression out of the tested combinations, but this part, along with BBa_J10051 and BBa_J10052, all shared the lowest expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. Figure 1. The highlighted points in yellow represent BBa_J10050 (weak promoter/ weak RBS) compared to BBa_J10051(weak promoter/ strong RBS) and BBa_J10052(weak promoter/ medium RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter. Antiquity UNIQc53a6d34c743b9c1-partinfo-00000003-QINU