Part:BBa_K3061002
Sp, signal peptide for Chlamydomonas reinhardtti
It is an N-terminal signal peptide and destined the protein to be either secreted, extracellular or periplasmic. They can be used for exogenous expression of proteins.
1. biology
Channelrhodopsin 2（ChR2） is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc（BBa_K3061010链接）around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):
Figure 1. Results of SignalP 4.1 server
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 52
Illegal NgoMIV site found at 61 - 1000COMPATIBLE WITH RFC[1000]
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