Coding

Part:BBa_K3061002

Designed by: Kaidi Chen   Group: iGEM19_DUT_China_B   (2019-10-16)
Revision as of 14:25, 21 October 2019 by Gwyndolin (Talk | contribs)


Sp, signal peptide for Chlamydomonas reinhardtti

It is an N-terminal signal peptide and destined the protein to be either secreted, extracellular or periplasmic. They can be used for exogenous expression of proteins.

1. biology

Channelrhodopsin 2(ChR2) is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc(BBa_K3061010链接)around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):

Figure 1. Results of SignalP 4.1 server

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None