Coding

Part:BBa_K3173010

Designed by: Thorge Dobbertin   Group: iGEM19_Ruperto_Carola   (2019-10-16)
Revision as of 14:24, 21 October 2019 by Thorged (Talk | contribs)

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STE2-Tail-EGFP

STE2-with v2 Tail, efficient TCS and NLS-EGFP. After addition of alpha mating factor TCS is accessible for Protease cleavage. Use e.g. BBa_K3173011 to tag alpha arrestin regions in the yeast genome with TEV protease or subclone BBa_K3173012 as an orthognal part to introduce cleavage function for Tango like assay into yeast cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1442
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 709


[edit]
Categories
Parameters
None