Composite

Part:BBa_K3002122

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 14:01, 21 October 2019 by Registry (Talk | contribs)

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Level 1 Mutant PETase + GLE + sp20-His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the SP20-His-tag (BBa_K3002018), the secretion signal GLE (BBa_K3002008) and the coding sequence of the Mutant PETase (BBa_K3002014) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1087
    Illegal PstI site found at 2060
    Illegal NgoMIV site found at 822
    Illegal NgoMIV site found at 849
    Illegal NgoMIV site found at 2551
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None