Composite

Part:BBa_K3002123

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 13:59, 21 October 2019 by Registry (Talk | contribs)

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Level 1 MHETase + cCA + sp20-His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the SP20-His-tag (BBa_K3002018), the secretion signal cCA (BBa_K3002007) and the coding sequence of the MHETase (BBa_K3002037) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
    Illegal NotI site found at 1662
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2572
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1651
    Illegal PstI site found at 1994
    Illegal PstI site found at 2804
    Illegal NgoMIV site found at 1260
    Illegal NgoMIV site found at 1721
    Illegal NgoMIV site found at 1751
    Illegal NgoMIV site found at 2066
    Illegal NgoMIV site found at 2084
    Illegal NgoMIV site found at 2120
    Illegal NgoMIV site found at 2763
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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