Part:BBa_K3009021

FPR2-receptor with mCherry

The human N-formyl peptide receptor 2 (FPR2) is a G-protein coupled receptor (GPCR) which is expressed on immune cell lineages like neutrophils and T-cells. Amongst other peptides, FPR2 senses the Staphylococcus aureus toxin PSMɑ3. It was proposed by Cheung et al (2014) that the PSMɑ3 FPR2 receptor interaction supposedly occurs via the formylated N- or the C-terminus of the peptide.[1] In response to receptor activation FPR2 elicits a signaling cascade depending on calcium ions as second messengers. Ultimately, this leads to immune cell activation, secretion of inflammatory cytokines and chemotaxis resulting in an inflammatory outcome. Neutrophil chemotaxis is triggered by PSM interaction with the FPR2 receptor [2] leading to an aggravation of the inflammation related symptoms in Staphylococcus aureus infection.

We provide here a BioBrick that can be used in signaling studies and the cellular detection of several small formylated amyloidogenic peptides. Commercially available peptide with inhibitory (WRWW4) and activating (WKYMVm) effect on FPR2 to test receptor activity without using a toxin.

The presence of FPR2 on transfected cells can be analyzed by fluorescence microscopy and flow cytometry via the attached mCherry reporter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1063
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]