Composite

Part:BBa_K3320019

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)
Revision as of 13:36, 21 October 2019 by Jocomodaro (Talk | contribs)


Lactate Repressor Unit

This is a part from our AND Gate, called lactate repressor unit.

Usage and Biology

In the presence of lactate, the PhlF Repressor protein is expressed, repressing the PhlF promoter with Tet repressor, allowing the production of GFPmut3 + HlyA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 965
    Illegal NheI site found at 988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3284


[edit]
Categories
Parameters
None