Part:BBa_K2992017

5-UTR containing RBS for fdx gene from C. sporogenes

5’-UTR containing RBS for fdx gene from C. sporogenes

Usage and Biology

Ferredoxin is an iron-sulfur protein involved in central redox reactions occurring during the general metabolism of Clostridia. 5’-UTR and RBS region for fdx is found naturally downstream of Pfdx and upstream of the start codon. In our project we use the regulatory region for the fdx gene comprising Pfdx-t14c (BBa_K2992016) and this entry to regulate our volatile reporter genes for predicting the production of botulinum neurotoxin following food manufacture.

Characterisation

Data incoming

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. (2019). ACS Synthetic Biology, [online] p. Available at: https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00075 [Accessed 21 Oct. 2019].

Minton, N., Ehsaan, M., Humphreys, C., Little, G., Baker, J., Henstra, A., Liew, F., Kelly, M., Sheng, L., Schwarz, K. and Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, pp.104-112.