Composite
Part:BBa_K3002126
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
Level 1 Wildtype-PETase + His
This composite part contains the PAR-promoter (BBa_K3002027), BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the His-tag a href="https://parts.igem.org/Part:BBa_K3002028">BBa_K3002028)and the coding sequence of the Wildtype-PETase (BBa_K3002004) plus the MoClo connectors for positions B2-B3 (BBa_K3002003), B4-B5 (BBa_K3002004)and B5-B6 (a href="https://parts.igem.org/Part:BBa_K3002005">BBa_K3002005).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1002
Illegal PstI site found at 1975
Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 764
Illegal NgoMIV site found at 2334 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |