Composite

Part:BBa_K3081057

Designed by: Song-Yuan Zhang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 13:16, 21 October 2019 by ZhangSongyuan (Talk | contribs)

CRISPR-based replication interference system for p15A plasmid copy number control, ini(+) site

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the bottom strand of initiation site on p15A origin. In this part, we add a degradation signal peptide "ssrA" to the dCas9 to alleviate its effect.

T--Peking--p15A_mechanism.png

Design of the p15A plasmid copy number control system

Mechanism of CRISPRri-based p15A plasmid copy number control

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 5495
    Illegal NheI site found at 5518
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

[edit]
Categories
Parameters
None