Part:BBa_K2992011

RBS region for botR gene from C. botulinum

RBS region for the botR gene in C. botulinum

Usage and Biology

This part entry represents the RBS from the alternative sigma factor-encoding gene botR. BotR is a positive regulator of neurotoxin production. In our project we use the botR RBS as part of the whole regulatory region for the expression of botR comprising PbotR and its native 5'-UTR containing the RBS (BBa_K2992014). This entry, the RBS was also used in conjunction with botR lacking its native promoter to allow for polar expression from the pyrD and pyrE genes on the C. sporogenes genome following genomic integration of botR .

Characterisation

Data incoming

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.

Zhang, Z., Korkeala, H., Dahlsten, E., Sahala, E., Heap, J., Minton, N. and Lindström, M. (2013). Two-Component Signal Transduction System CBO0787/CBO0786 Represses Transcription from Botulinum Neurotoxin Promoters in Clostridium botulinum ATCC 3502. PLoS Pathogens, 9(3), p.e1003252.