Part:BBa_J10050:Experience
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Applications of BBa_J10050
User Reviews
UNIQe850285a091058d9-partinfo-00000000-QINU
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ETH Zurich 2014 |
Characterization of weak promoter (BBa_J23113) and weak RBS (BBa_B0031)Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10050 would result in the least amount of expression out of the tested combinations, but this part, along with BBa_J10051 and BBa_J10052, all shared the lowest expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
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