Part:BBa_K3142014

PT-αcrp + acmA

what it is：

what it does：

how to use it in their projects：

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 26
  Illegal XbaI site found at 729
  Illegal PstI site found at 253
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 26
  Illegal PstI site found at 253
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 26
  Illegal BamHI site found at 1
  Illegal BamHI site found at 681
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 26
  Illegal XbaI site found at 729
  Illegal PstI site found at 253
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 26
  Illegal XbaI site found at 729
  Illegal PstI site found at 253
  
• 1000
  COMPATIBLE WITH RFC[1000]

SZPT-China

To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 1. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 2. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.

Reference

• William Henry Bothfeld, Grace Kapov, and Keith Tyo. A glucose-sensing toggle switch for autonomous, high productivity genetic control. ACS Synth. 2017, 6(7):1296-1304.