Composite

Part:BBa_K2974400:Design

Designed by: Janet Standeven, Megan Hong, Courtney Taing, Julia Chang, Shanthi Hegde, Sri Pinnamareddy   Group: iGEM19_Lambert_GA   (2019-10-16)
Revision as of 12:46, 21 October 2019 by Courtneytaing (Talk | contribs)


T7 C. elegans Trigger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 159
    Illegal NotI site found at 6
    Illegal NotI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69


Design Notes

T--Lambert GA--Lin4Megan.png

Figure 1: BLAST results for C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)




T--Lambert GA--ToeholdMegan1.png

Figure 2: Complementary trigger RNA sequence derived from C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)


Source

The lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic) is native to the genome of C. elegans.

References