Coding
Part:BBa_K3113008
Designed by: Theresa Keil Group: iGEM19_Munich (2019-09-04)
Revision as of 12:37, 21 October 2019 by Theresakeil (Talk | contribs)
HiBiT
HiBiT is a peptide tag used for antibody-free endogenous protein detection. It is 11 amino acids long and binds with high affinity to another larger subunit called LgBiT. The bound complex has luciferase activity. Promega has all rights to this sequence.
Usage
Biology
“The Nano-Glo® HiBiT Lytic Detection System quantifies cellular protein in minutes with high sensitivity and a broad dynamic range using a simple add-mix-read assay format.”[1] The HiBiT peptide is an 11 amino acid long tag that can be added to the protein of interest. The tagged protein can be measured with the Nano-Glo® HiBiT Detection Reagent which contains the LbBiT. Together HiBiT and LgBiT form a functioning luminescent NanoBiT® enzyme.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References/h2>
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Categories
Parameters
| None |