Signalling
Subtilin P

Part:BBa_K104001:Experience

Designed by: The Newcastle 2008 iGEM team   Group: iGEM08_Newcastle_University   (2008-10-24)
Revision as of 10:25, 25 October 2008 by Mtaschuk (Talk | contribs) (→‎Applications of BBa_K104001)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K104001

The part was cloned into the Bacillus integration vector pJWV1012 upstream of both gfp and mCherry.

It was then integrated into the chromosome of Bacillus subtilis 168.

We showed that 168 was able to sense the levels of subtilin quorum communication peptide (SpaS product) in the external media.

The part can therefore be used as a generic sensor for subtilin mediated communication between Gram positive bacteria.

An ideal addition would be a resistance brick. Without the resistance genes, subtilin will kill Bacillus subtilis 168, causing cell lysis at high concentrations.

User Reviews

UNIQ196b9162dc084326-partinfo-00000000-QINU UNIQ196b9162dc084326-partinfo-00000001-QINU