Part:BBa_K2926054

Fusion protein of mating factor alpha from yeast, mCherry and pVIII from the M13 bacteriophage

Mating factor alpha was N-terminally fused to the red fluorescent protein mCherry. Additionally the major coat protein pVIII of the bacteriophage M13 was fused to the N-terminus of mCherry.

Usage and Biology

This part was used during Troygenics Assembly to visualize the produced M13 bacteriophage-derived Troygenics via fluorescence measurement (Fig. 1).

Fig. 1: Emission spectrum of our mCherry-marked Toygenics with mCherry as a comparison measured with TECAN infinite M200 plate reader(λ[Ex]=570 nm, λ[Em]=600 nm to 850 nm).

We compared the emission spectrum of our Troygenics with the emission spectrum of mCherry, because the fluorescence marker cloned on the Troygenics is mCherry, too. We have seen a difference in the emission peak of about 20 nm. This is probably because the mCherry on our Troygenics is fused to the whole Troygenic. Because of this, there might be a different folding resulting in a shift of the emission spectrum.

Sequence and Features

Sequence was validated via Sanger sequencing.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]