![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K108025:Design
Designed by: Qi Liu Group: iGEM08_Tsinghua (2008-10-23)
Revision as of 08:27, 25 October 2008 by Angel6767Q (Talk | contribs)
Pu
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 163
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
Source
Pu was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).
References
1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.
2. NCBI.