Part:BBa_K3021006

pilA-Laccase1326-HisTag

This is a new composite part designed to allow Laccase secretion from our engineered cyanobacteria through embedded the secretion peptide, pilA1. Laccase is an important enzyme, which would degrade different chemicals. The Laccase sequence in this part is based on the laccase1326 cDNA sequence from previous study, which was shown to have great tolerant to different harsh conditions commonly found in polluted water. Secretion of heterologous proteins into cell culture medium offers significant advantages for downstream processing over production. pilA1 is the commonly know with its effect on the cyanobacteria secretion.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1014
  Illegal XhoI site found at 234
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 869
  

Sequencing and sequence alignment of K3021006

Figure 1. Representative sequencing results of K3021006_pSCBe (pilA_Lac1326_pSCBe)

To future confirm that our constructs have correct sequence, we send our clones (with positive PCR results) out to sequencing.

The sequencing results show that we have successfully assembled target genes K3021006 (pilA_Lac1326_6xHis) to the vector (pSCBe).