Composite

Part:BBa_K2924052:Design

Designed by: Melanie Sbielut   Group: iGEM19_Duesseldorf   (2019-10-16)
Revision as of 11:50, 21 October 2019 by Mesbi100 (Talk | contribs) (→‎References)


Hpall + SPNprE + alpha s2 + fd terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 554


Design Notes

Expression system for the expression and secretion of the protein in B. subtilis

Source

PBSMul1 plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s1-casein. a-s1-casein was commercial synthesized by GenScript.

References

Brockmeier, U., et al. (2006). "Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria." J Mol Biol 362(3): 393-402.

Knapp, A., et al. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.