Composite

Part:BBa_K2924033:Design

Designed by: Melanie Sbielut   Group: iGEM19_Duesseldorf   (2019-10-16)
Revision as of 11:48, 21 October 2019 by Mesbi100 (Talk | contribs) (→‎Source)

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Hpall + SPNprE + α-s1-casein + fd terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Expression system for the expression and secretion of the protein in B. subtilis

Source

PBSMul1-SPNprE plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s1-casein. a-s1-casein was commercial synthesized by GenScript.

References

Brockmeier, U., et al. (2006). "Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria." J Mol Biol 362(3): 393-402.

Knapp, A., et al. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.