DNA

Part:BBa_K3046032:Design

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)
Revision as of 11:44, 21 October 2019 by Lookingforanswer (Talk | contribs) (→‎Design Notes)

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IS2 upstream sequence for A.niger genome integration


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal XbaI site found at 1623
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal BamHI site found at 1729
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal XbaI site found at 1623
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal XbaI site found at 1623
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It should be possible to de novo synthesize this part. Generally the part does not contain too many repeats, and we have had no problems with designing primers for PCR/sequencing that binds in this sequence

Source

Our IS2 parts were created by Dorthe Holm (2013) as part of a ph.d. thesis: Holm, D. K. (2013). Development and Implementation of Novel Genetic Tools for Investigation of Fungal Secondary Metabolism. Kgs. Lyngby: Technical University of Denmark.


References