Composite

Part:BBa_K2926086

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-21)
Revision as of 11:43, 21 October 2019 by Jopgenoorth (Talk | contribs)

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Basic Insert to express Cas13a (Lwa) and a short guideRNA array in S. cerevisiae

This part enables the expression of Cas13a (Lwa) and a guide RNA array containing three guide RNAs that target essential genes in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4719
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1730
    Illegal BglII site found at 3410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4694
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2809


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Categories
Parameters
None