Composite

Part:BBa_K2926084

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-21)
Revision as of 11:37, 21 October 2019 by Jopgenoorth (Talk | contribs)

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Basic Insert to express Cas13a (Lbu) and a guideRNA array in S. cerevisiae

This part enables the expression of Cas13a (Lbu) and a guide RNA array containing seven guide RNAs that target essential genes in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4564
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 788
    Illegal BglII site found at 1724
    Illegal BglII site found at 1988
    Illegal BglII site found at 2492
    Illegal BglII site found at 2978
    Illegal BglII site found at 3086
    Illegal BglII site found at 3416
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4539
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3097


[edit]
Categories
Parameters
None