Regulatory

Part:BBa_K3007023:Design

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)
Revision as of 11:30, 21 October 2019 by Leaves (Talk | contribs)


CP6 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 32
    Illegal XbaI site found at 135
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 32
    Illegal PstI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 32
    Illegal BamHI site found at 43
    Illegal BamHI site found at 118
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 32
    Illegal XbaI site found at 135
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 32
    Illegal XbaI site found at 135
    Illegal PstI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we used the part, the sequence at the 5' end of this part in the original vector (cctgatgcggtattttctccttacgcatctgtgcggtatttcact) was retained.


Source

E. coli

References