Coding

Part:BBa_K3098008

Designed by: Chenyi HE   Group: iGEM19_WHU-China   (2019-10-01)
Revision as of 11:29, 21 October 2019 by Chenyihe (Talk | contribs)


GST+PgD5

In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence . GST tag can help the peptides dissolve in cytoplasm to help the expression of the peptide.During purification, GST tag can bind with the corresponding column to keep the target protein when the impurities leave. After washing, PreScission Protease (also have GST tag) will be added to cut the GST tag from the target protein, in this way, we'll be able to collect pure solution of the target protein without adding any more impurities.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


[edit]
Categories
Parameters
None