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Part:BBa_K2974400:Experience

Designed by: Janet Standeven, Megan Hong, Courtney Taing, Julia Chang, Shanthi Hegde, Sri Pinnamareddy   Group: iGEM19_Lambert_GA   (2019-10-16)
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T7 C. elegans Trigger
The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the trigger. In addition, sequencing analysis results shown below also confirms the presence of the trigger in comparison to the trigger sequences in the reference paper (Green et al. 2014). The biobricked trigger's (BBa_K2974400) function has been validated through experimentation and sequencing.


Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.

Sequencing Analysis


Figure 2: Lambert iGEM aligned the Trigger insert with the Eurofins Genomics sequencing results. This showed that the Trigger was successfully cloned.

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