Composite

Part:BBa_K3110003:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 10:47, 21 October 2019 by Suryazzz (Talk | contribs) (→‎Design Notes)

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lldP + lldR + lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3597
    Illegal AgeI site found at 411
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1471
    Illegal AgeI site found at 1483
    Illegal AgeI site found at 2229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3034


Design Notes

The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.

Source

lldD was synthesized by Twist Biosciences and lldP + lldR was obtained from the genomic DNA of Ecoli Dh5α. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR).

References