Coding

Part:BBa_K108009:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)
Revision as of 05:28, 25 October 2008 by Angel6767Q (Talk | contribs) (→‎References)

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FliK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 483


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

FliK was cloned from E.coli K12 DH10B genome (reference 1).

References

1. NCBI.