Coding

Part:BBa_K108005:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)
Revision as of 05:03, 25 October 2008 by Angel6767Q (Talk | contribs) (→‎Source)

FlgH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

FlgH was cloned from E.coli K12 DH10B genome (reference 1).

References