Part:BBa_K3061002

Sp, signal peptide for Chlamydomonas reinhardtti

It is an N-terminal signal peptide and destined the protein to be either secreted, extracellular or periplasmic. They can be used for exogenous expression of proteins.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 52
  Illegal NgoMIV site found at 61
  
• 1000
  COMPATIBLE WITH RFC[1000]

1. biology

Channelrhodopsin 2（ChR2） is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc（BBa_K3061010链接）around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):

Figure 1. Results of SignalP 4.1 server