Coding

Part:BBa_K2926001

Designed by: Ina Schmitt   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)
Revision as of 09:32, 21 October 2019 by Jopgenoorth (Talk | contribs)


Cas13a Lbu

This part codes for Cas13a derived from Leptotrichia buccalis. The natural EcoRI and PstI restriction sites were removed via mutagenesis PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 344
    Illegal BglII site found at 1280
    Illegal BglII site found at 1544
    Illegal BglII site found at 2048
    Illegal BglII site found at 2534
    Illegal BglII site found at 2642
    Illegal BglII site found at 2972
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2653


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Categories
Parameters
None