Reporter

Part:BBa_K3137011

Designed by: Jiamin Chen   Group: iGEM19_JiangnanU_China   (2019-10-07)
Revision as of 08:39, 21 October 2019 by LWJ (Talk | contribs)


gfp

The gfp can express green fluorescent protein, which can be used as a marker gene in the process to report the number of cells.

Usage and Biology

When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to display green fluorescence.


Characterization of PputA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None