Reporter

Part:BBa_K3137004

Designed by: Jiamin Chen   Group: iGEM19_JiangnanU_China   (2019-10-07)
Revision as of 08:36, 21 October 2019 by LWJ (Talk | contribs)


mCherry

The mCherry can express red fluorescent protein and it is used as a marker gene in the process of reporting the number of cells.

Usage and Biology

When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF will induce expression of the red fluorescent protein gene mCherry(BBa_K3137004), which will make the bacteria display red fluorescence.

Characterization of PglcF

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None