Part:BBa_K3137012

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VgrG is from Escherichia coli strain, which is normally considered to be a secreted protein.

Usage and Biology

To get more efficient phage resistant parts, we used the ARTP(Atmospheric and room temperature plasma) mutagenesis system and Comparative Genomics to obtain 4 mutant bacterial and 4 key mutant sites (nuoE, yhjH, rzpD And gntR).Resistance tests on these key sites were performed respectively(Fig 1).

Figure 1. Resistance test for T4 phage of NuoE, YhjH, RzpD and GntR

Since it is not possible to directly see from the figure which component has the least influence on the growth of the bacteria, we use the Grey Relation Analysis (GRA) method to analyze the growth curve (Fig 2) of the bacteria connecting the various components.

Figure 2. Effect of different anti-phage parts on the growth of E. coli BL21

We used the Entropy Weight Method (EWM) to determine the weight of each growth point to select the most similarly modified strain (the highest correlation), which is the component that has the least impact on bacterial growth. At the same time, after consulting the industry experts, we revised the weights according to the experts' recommendations to evaluate the components again, making them more in line with the real situation of production, that is, the most suitable components for industrial production. Using GRA's two evaluations of the four components at different weights.(Fig 3)

Figure 3. EWM weight & advice weight and the results of GRA for selecting most suitable strain

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]