Composite
Part:BBa_K3078203:Design
Designed by: Ziang Guo Group: iGEM19_Jilin_China (2019-10-20)
T4 holin regulated by 4-HPA sensing system.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1010
Illegal BglII site found at 1476
Illegal BglII site found at 1905
Illegal BamHI site found at 2406 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 628
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When culturing engineering bacteria containing this part, it is necessary to have 4-HPA in the environment all the time.
Source
This part comes from our project.