Part:BBa_K1033916:Experience
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UNIQ87d21a98454f1573-partinfo-00000000-QINU UNIQ87d21a98454f1573-partinfo-00000001-QINU
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Hong Kong-CUHK iGEM 2017 |
Fluorescent properties of amajLime Although amajLime is described as chromoprotein in main page, we characterised its spectral properties and found the max excitation and emission wavelength at 445 nm nd 485 nm respectively.
We transformed part BBa_K1033916 with constituitive promoter: J23100 in C41 and grew in 2XYT for 24 hours. Purifying the amajLime by Ion Exchange Chromatography and Hydrophobic Interaction Chromatography, we measured the fluoresece of purified amajLime, which is diluted to 10µg/100µl (total 200µl) in triplicates, in different buffers (ranges from pH2 to pH12). The result shows that the stability drops dramatically in pH condition below 6 and attains maxima fluorescence at pH 8. |