Part:BBa_K3145001:Design

sfGFP (Super-Folder Green Fluorescent Protein)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

There is C-terminal strep tag

Source

The sfGFP sequence originated from our original pY71-sfGFP construct which was made using the Kwon & Jewett 2015 journal article in our references.

References

• Kwon, Y.-C. & Jewett, M.C.High-throughput preparation methods of crude extract for robust cell-free protein synthesis. Sci. Rep. 5,8663; DOI:10.1038/srep08663 (2015).

• Bundy, B. C., and Swartz, J. R.Site-Specific Incorporation of p-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation. [https://pubs.acs.org/doi/10.1021/bc9002844#%7CBioconjugate

Chem.21 (2), 255-263;DOI: 10.1021/bc9002844