Regulatory

Part:BBa_K3031015

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-15)
Revision as of 05:32, 21 October 2019 by Davystar (Talk | contribs)


LuxI with FUR box located in the -10 region

This is the LuxI promoter sequence with a standard Ferric Uptake Regulator sequence (FUR box) inserted between the -35 and -10 region. The FUR box allows for the repression of Quroum Sensing expression of genes associated in the presence of ferric iron. In the classical FUR repression mechanism, iron-bound Fur binds to a Fur box sequence that overlaps with, or is proximal to, promoters of iron responsive genes, thus preventing their transcription. When intracellular iron is depleted, Fe2+ is released from Fur, causing conformational changes in the protein resulting in dissociation from the Fur box.

In a low iron environment, through the regulation of the LuxI-Fur box promoter, the LuxI protein (AHL) would be produced as normal which forms an integral part of the well characterized and used LuxR/LuxI quorum sensing system.

In the absence of Fe the downstream genes are once gain turned on at high cell densities.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None