Part:BBa_K2440005

SV40 promoter

This is a promoter gene derived from SV40. It could initiate the transcription of downstream gene.

Usage and Biology

The promoter is a DNA region that initiates the transcription of a particular gene. The promoter is located upstream of the same strand at the transcription initiation site of a particular gene. ¹

At pmirGLO Vector, SV40 early enhancer/promotor is located at 426–844 nucleotides, with a length of 419 nucleotides. Its role is to initiates the transcription of the hRluc-neo fusion protein coding region.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Contribution:NUDT_CHINA, 2019

Summary: We add experiment data to the part regard to the promoter’s strength at continous time set after transfection of 0, 06, 12 and 24 hours. We transfered 500ng pmirGLO with the gene of SV40 promoter and hRluc into each well of 24 well plate of HepG2 by lipo3000 and detected its RLU via Dual Luciferase Reporter Gene Assay Kit. To show the result clearly, we made a bar chat as follows.

Reference

[1] Gagniuc P, Ionescu-Tirgoviste C. (Apr 2013)."Gene promoters show chromosome-specificity and reveal chromosome territories in humans" BMC Genomics. 14:278. PMID: 23617842 PMCID: PMC3668249 DOI: 10.1186/1471-2164-14-278