Composite

Part:BBa_K3139014

Designed by: Jiapei Han   Group: iGEM19_NAU-CHINA   (2019-10-16)
Revision as of 04:38, 21 October 2019 by Thousandwinds (Talk | contribs) (→‎Usage and Biology)


J23100-fusion protein-B0015-J23106-TEVp-TEVp site-HasA-B0010 anti-plasmodium system


Usage and Biology

In the anti-plasmodium system of NAU-2019, the amount of both TEV protease BBa_K3139012and anti-plasmodium fusion protein BBa_K3139013should be kept at a certain proportion to avoid a waste of cell resources. If the TEV protease is excessive, it shall be wasted though the fusion proteins are cleaved completely. If the fusion protein is excessive, it cannot be cleaved completely with insufficient TEV protease. By testing different promoter combinations of TEVp and fusion protein, we tried to find out the most economical combination which has the least waste and most complete cleavage with the help of mathematic model. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2232
    Illegal NheI site found at 2255
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 444
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2582
    Illegal SapI.rc site found at 2930


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