Coding

Part:BBa_K3170996

Designed by: Haodong Yan   Group: iGEM19_LZU-CHINA   (2019-10-16)
Revision as of 04:16, 21 October 2019 by Studentwang (Talk | contribs)


TEVp

Tobacco etching virus protease, Part of the protease-protease regulation system

  • TEV Protease is a recombinant protease derived from Tobacco Etch Virus (TEV) Nla, which is used to excise the affinity tag of the purified fusion protein. TEV protease has strong site specificity and can recognize the seven amino acid sequence of EXXYXQ(G/S) (Glu-Asn-Leu- Tyr-Phe-Gln-Gly). The most common one is ENLYFQG, and its cleavage site is at Between glutamine and glycine or serine.

Experimental Results

T--LZU-CHINA--TEVp.png
  • Using the specificity of TEVp, we cleave the recognition sites of ALKBH (Fig.A) and Casp3 (Fig.B) linked by the target sequence, which are active.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None