Composite

Part:BBa_K2575000

Designed by: Stephan George   Group: iGEM18_UGA   (2018-10-10)
Revision as of 02:03, 21 October 2019 by LuiSuzukiWilliams (Talk | contribs) (→‎Characterization: UGA 2019)


BS3 Resistance Gene, plant reporter under control of Gal1P

The BS3 resistance gene is an avirulence initiator protein from Capsicum annuum that promotes cell death in response to its activation. This part is attached to a Gal1P to enable activation in response to Gal4, thus enabling easy activation in plant lines through co-transformation with Gal4. The BS3 resistance gene encodes a flavin monooxygenase that is activated by AvrBS3, and results in plant hypertrophy to stop the invading pathogen. This hypertrophy can be used as a reporter of gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization: UGA 2019

The 2019 UGA iGEM team cotransformed this part with a GAL4BD-VP16 transcription factor into agrobacterium. Upon a successful transformation, the team transfected Nicotiana benthamiana and observed the expression of the BS3 resistance gene.

In the figures that follow, the results of the transfection of GAL4BD-VP16 and 1XUAS-BS3 into Nicotiana benthamiana can be seen. For each plant sample, multiple experimental conditions were tested. Figure 4 displays and codifies each experimental condition.

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