Part:BBa_K157001:Design

EGFR/ErbB1 signal peptide; mediates protein transport to a translocational pore

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3
  

Design Notes

This sequence mediates transportation of the protein to a translocational pore by an RNA–multiprotein complex, the signal recognition particle (SRP) when fused to the n-terminus of a fusion protein with a transmembrane region (Walter P, Johnson AE: “Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane.” Annu Rev Cell Biol 1994, 10:87-119). There, after a pause of translation, the signal sequence is released and translation and translocation of the nascent chain are restarted (Robert M Stroud, Peter Walter: “Signal sequence recognition and protein targeting”, Current Opinion in Structural Biology 1999, 9:754–759).

Source

Human EGFR ErbB-1 signal sequence, originally mediating membrane integration of the EGF-receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533; DNA synthesis and optimization by GeneArt.

References

-Human EGFR ErbB-1 signal sequence, originally mediating membrane integration of the EGF-receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533; DNA synthesis and optimization by GeneArt.

-Robert M Stroud, Peter Walter: “Signal sequence recognition and protein targeting”, Current Opinion in Structural Biology 1999, 9:754–759