Coding
Sod Cu/Zn

Part:BBa_K847004

Designed by: Rashmi Sharma, Vishesh Jain, Kendrick Wang   Group: iGEM12_Stanford-Brown   (2012-08-09)
Revision as of 01:30, 21 October 2019 by Kparkhurst (Talk | contribs)

''Deinococcus radiodurans'' Superoxide dismutase, SodCu/Zn

Deinococcus radiodurans is an extremely radiation-resistant bacterium: while about 10 Gy (absorbed radiation dose, Gray) can kill most vertebrates, D. radiodurans can withstand up to 12,000 Gy. Current literature supports this finding with D. radiodurans’ unique genetic makeup which allows it to better handle radiation exposure. The two main effects of radiation exposure to bacterial cells are DNA damage and the creation of toxic superoxide species (Daly 2009). To take care of the pesky superoxides, superoxide dismutases are expressed in high levels in D. radiodurans (Slade and Miroslav 2011). These enzymes break the reactive species down into harmless oxygen and hydrogen peroxides. This dismutase uses copper and zinc as cofactors (Gao, Zhang, Song, Chen, and Zhong 2009).

RHIT 2019’s Characterisation

Characterization of BBa_K847004, the protocol for which can be found in the lab notebook, shows that bacteria containing the SOD gene in this experiment have better survivability against the reactive oxygen species produced by copper, including a larger number of final CFUs, as well as survivability at higher concentrations of copper total.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
protein