Reporter

Part:BBa_K3105670

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)
Revision as of 01:15, 21 October 2019 by JonasG (Talk | contribs) (→‎Usage)

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eGFP

enhanced green-fluorescent protein codon optimised for Pichia pastoris without start-codon

Usage

eGFP was used as a reporter gene for assessing recombinant protein expression in P. pastoris. For this purpose, C-terminal fusions were created. Examples for this usage are the Parts BBa_K3105677 and BBa_K3105680.
Functionality of this part in P. pastoris was shown in BBa_K3105680.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None