Coding

Part:BBa_K3105668

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-09-25)
Revision as of 01:13, 21 October 2019 by JonasG (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Horseradish peroxidase

Coding sequence of horseradish peroxidase (HRP) C1A codon optimised for Pichia pastoris.

HRP is commonly used as a antibody-conjugate and is detected by chemiluminescence.

The underlying reaction mechanism is described below:
HRP+ H2O2 = oxidized substrate


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 961
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None